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Fig. 4. Distribution of Hsc70 immunofluorescence in unfertilized eggs. These images
are examples of two eggs out of 25 observed. (A,B) Examples of diffuse
cytoplasmic distribution of Hsc70 and exclusion from the nucleus in two eggs
incubated in 20 or 5 µg Hsc70, respectively. In each set of panels, 1, 2
and 3, and 1', 2' and 3' are corresponding DIC and
fluorescence optical sections. Section 2' in each panel is through the
center of the nucleus (arrow), whereas 1' and 3' are 1.5 µm
above and below that, respectively. (C) An enlargement of one area of an egg
treated with 5 µg Hsc70 for 30 min, illustrating the absence of
correspondence between locations of higher Hsc70 immunofluorescence and
vesicles. The left and right panels are, respectively, the DIC and
fluorescence images of identical regions. The solid circles enclose examples
of vesicles in the DIC image and the corresponding areas in the fluorescence
image, showing that higher fluorescence intensity does not co-localize with
vesicles. Conversely, two locations of bright fluorescence enclosed by the
broken circles in the right panel do not co-localize with vesicles in the left
DIC image. The center pseudocolored rendition of the fluorescence image was
created to confirm that the circles correspond exactly to the same areas in
the two images. In this image, blue represents low and red represents high
fluorescence intensity. The gray oval is a portion of the DIC image rendered
semitransparent and superimposed over the identical area in the pseudocolor
image to confirm correspondence.
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