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Figure 3


Fig. 3. Renaturation of luciferase in the presence of Hsc/Hsp70 or Hsc70 as a measure of their refolding activities. 2 µg ml–1 of Hsp70 and of Hsc/Hsp70 was heated in the presence of luciferase to determine the ability of the Hsp preparations to prevent luciferase denaturation. The heated luciferase–Hsp mixture was combined with luciferin reagent and luminescence recorded as a percentage of unheated luciferase. The difference in phosphorescence between luciferase heated in the absence of Hsp and in the presence of Hsp was statistically significant as indicated by the asterisk (P<0.05). There was no difference in effectiveness between the two Hsp preparations. Values are means ± s.e.m. N=6 replicate experiments.





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