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Fig. 1. The Primary (pre-treatment) and Secondary (post-treatment) humoral immune
responses (means ± s.d.) to tetanus and diphtheria antigens in red
knots subjected to different Experimental Treatments. The treatment was almost
identical for all birds up to the Primary response (see text). The week before
the Secondary response was measured, Flyers (N=6) flew the equivalent
of 1500 km in a wind tunnel. Failed Flyers (N=5) were birds
originally assigned as Flyers that refused to fly for long periods in the wind
tunnel. Controls (N=8) did not fly in the wind tunnel. The long
flights had no detectable effect on the humoral immune response of the birds.
Humoral immune responses were measured as the antigen-specific antibody
response using a kinetic ELISA. A higher antibody titer against a specific
antigen was detected as a faster colour change over time [measured in milli
Optical Densities (mOD) min1], which is equivalent to more
antibodies specifically bound to the antigen.