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Fig. 4. Exposure to 30%SW significantly changes the Michealis–Menten kinetics
of Na+ and Cl– influx at the papillae.
Na+ or Cl– influx was calculated using the
measurements obtained from the SIET system from a single point on the surface
of the anal papillae while changing the bath NaCl concentration. The results
obtained from each individual papilla were fitted to the
Michaelis–Menten equation using non-linear regression analysis.
Jmax, maximum rate of transport; Kt,
Na+ or Cl– concentration that yields a flux of 50%
Jmax. (A) Na+ and (B) Cl–
influx from single papillae obtained from larvae maintained in FW (freshwater;
squares) or in 30%SW (seawater) for 5 h (triangles). (C)
Kt and (D) Jmax (means ±
s.e.m.) for groups of papillae obtained from larvae that were either held in
freshwater (FW), had been transferred from FW to 30%SW for 5 h (30%SW), or
were held in 30%SW for 5 h and subsequently transferred to FW (30%SW/FW) for
20 h (Na+, white bars, N=6–13) or 5 h
(Cl–, black bars, N=8–13). An asterisk denotes
a statistically significant difference from the FW group. Kinetic parameters
for Na+ (N=6) and Cl– (N=5)
uptake by the papillae of intact larvae (in vivo) held in FW are also
shown (no difference from FW, t-test, P<0.05).
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