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Figure 1


Fig. 1. Spatiotemporal maps (STMap) of the luminal flow were constructed from unfed larvae at 4 and 7 d.p.f. (A) A box was drawn around the area of interest, covering most of the gut (~900 µm). PI, proximal intestine (intestinal bulb); MI, middle intestine; DI, distal intestine. (B) STMaps, over the area within the box in A, were created based on changes in gut opacity as the gut content was propelled in a retrograde or anterograde direction. Individual contractions are identified as darker bands, travelling in either direction over time (downwards in map, ~3.8 min). In this STMap, six anterograde contractions waves are present. Velocity (v, the slope of the line), interval between consecutive contractions ({Delta}t), distance (d) travelled by the contractions and the total time of activity were calculated. Asterisks indicate the region where the contraction waves originated.





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