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Fig. 4. Quantitative estimation of nuclear translocation of phosphorylated ERK in
RTgill-W1 cells, as determined from the ratio of staining intensity measured
in the nucleus to that of a cytoplasmic region of the cells. (A) Time course
of the change in nuclear/cytoplasmic ratio following the addition of EGF is
shown in controls and in cells after pre-incubation with leptomycin B. Values
are means ± s.e.m. of at least 20 cells from 2–3 independent
cultures. (B) Nuclear/cytoplasmic fluorescence ratio of cells after exposure
to hypo-osmotic shock, EGF or Cu at the time where the maximum increase of
relative nuclear fluorescence was determined (5' or 15'). Values
are means ± s.e.m. of at least 16 cells from 2–3 independent
cultures. Asterisks indicate a statistical significant difference
(P<0.05) from the value at time zero (A) or from cells in the
absence of LB (B).
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