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Fig. 7. Activity levels of the selected and control flies during the morning
(05:00–09:00 h) and evening (17:00–21:00 h) windows of selection.
(A) Percentage activity during the morning (M) window in the selected and
control populations. (B) Percentage activity during the evening (E) window in
the selected and control populations. Error bars represent 95% confidence
intervals (95%CI) around the mean for visual hypothesis testing. The average
locomotor activity plots of the (C) early (N=161), (D)
control (N=171) and (E) late (N=156)
flies, monitored under a 12:12 h LD cycle after 55 generations of selection.
Locomotor activity profiles are plotted as the mean activity during 1 h bins,
averaged over 10 consecutive cycles. The percentage of activity, averaged over
10 successive cycles, is plotted along the ordinate and time of the day (h)
along the abscissa. Values are means ± s.e.m., constructed using the
variations among the replicate populations within each selection regime. The
white and grey vertical bars denote activity levels during the day and night,
respectively. Additionally, one representative locomotor activity pattern each
of flies from the (F) early, (G) control and (H)
late populations are shown. The horizontal black bars denote the dark
phase (20:00 h–08:00 h) and white bars represent the light phase of the
LD cycle. Zeitgeber time 0 (ZT0) denotes the time at which lights come on
under the LD cycle. The average locomotor activity plots of the (I)
early (N=32), (J) control (N=27) and (K)
late (N=37) flies, during the first cycle of DD following a
LD/DD transfer. Horizontal dark grey bars denote the subjective night and
light grey bars the subjective day under DD conditions. Vertical dark and
light grey bars denote activity during the subjective night and subjective
day, respectively, under DD.
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