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Fig. 1. Schematic representation of the selection protocol. The selection
experiments were carried out under 12:12 h light:dark (LD) cycles [`lights-on'
at 08:00 h and `lights-off' at 20:00 h, where Zeitgeber Time 0 (ZT0) denotes
lights-on]. Four baseline populations (baseline1..4)
maintained for over 75 generations under 12:12 h LD cycles were used to derive
four early (early1..4) and four late
(late1..4) populations of flies by imposing selection for
early and late adult emergence. Four control populations
(control1..4) were also raised in a similar manner, except
that they did not experience any conscious selection pressure. Flies emerging
between 05:0009:00 h formed the early populations, while those
emerging between 17:0021:00 h made it to the late populations.
Flies emerging through out the day were used to raise the control
populations. The morning (M) and evening (E) selection windows are shown in
the grey boxes in the early and late panels.