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Figure 8


Fig. 8. Detection of APSGFLGMRamide (CabTRP Ia) in the hemolymph of unfed and fed Cancer productus using MALDI-FTMS. To assess whether or not circulating levels of Gly1-SIFamide and/or CabTRP Ia are influenced by the feeding status of an individual animal, hemolymph samples were collected and analyzed from animals held without food for approximately seven days (A), as well as from those held without food for approximately one week but allowed to feed at will for 2 h prior to sampling (B). As can be seen in the representative spectra, a peak corresponding to the [M+H]+ ion for CabTRP Ia, i.e. m/z 934.49, was detectable only in the hemolymph of unfed animals. Gly1-SIFamide was not detected in spectra from either unfed or fed individuals. With the exception of the inset, both A and B are shown at the same (m/z) scale.





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