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Fig. 5. SIFamide- and tachykinin-related peptide-like immunopositive endocrine
cells are differentially distributed within the Cancer midgut
epithelium. In each of the species investigated (Cancer borealis
shown), the cells labeled by the SIFamide and substance P antibodies were
differentially distributed. Specifically, the SIFamide (SIFa)-stained cells
(pseudocolored green) were restricted to the epithelium of the anterior
portion of the midgut proper and the anterior midgut caeca (shown in A),
whereas substance P (Sub P) immunopositive cells (pseudocolored red) were
concentrated in the posterior portion of the midgut proper and the posterior
midgut caecum (shown in B). As the few substance P-like immunopositive cells
seen in the anterior midgut (arrows) were not among those labeled by the
SIFamide antibody, and vice versa (e.g. the red and green, but not
yellow, cells present in A), no colocalization of the two peptides is apparent
in the midgut. It should be noted that some epithelial endocrine cells possess
a short, thin, basal process that projects along the outer surface of the
midgut (arrows in B). This type of projection was seen in a subset of both the
SIFamide- and TRP-like immunopositive cells (TRP cell shown). A1
(anti-SIFamide) and A2 (anti-substance P) are brightest pixel projections of
28 optical sections collected at 1.05-µm intervals. Both labels were imaged
simultaneously. A3 is a merge of A1 and A2. B1 (anti-SIFamide) and B2
(anti-substance P) are brightest pixel projections of 26 optical sections
collected at 1.05-µm intervals. Both labels were imaged simultaneously. B3
is a merge of B1 and B2. A1, A2, B1 and B2 are all shown at the same scale,
with the scale bar in B2 equal to 200 µm. A3 and B3 are shown at the same
scale, with the scale bar in B3 also representing 200 µm.
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