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Figure 5


Fig. 5. Synchronization of rhodopsin promoter expression in individual rod photoreceptor cells. (A) Rhodopsin promoter-driven GFP expression in 24 h in constant darkness (DD), except at 22:00 h, when a 30-min light pulse was applied. Exposure to light (indicated by the arrow) synchronized the expression in all rod cells (N=10). After light treatment, the expression began to increase. (B) Light exposure (N=21) or the application of dopamine (N=16) or dopamine D2 receptor agonist quinpirole (N=14) decreased rhodopsin promoter expression. In the presence of the dopamine D2 receptor antagonist sulpiride, light produced no effect on rhodopsin promoter expression (N=19). Each line represents time-lapse imaging data from one rod cell. Horizontal lines represent the duration of treatment.





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