|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 8. Expression of Hsp70 mRNA in isolated lateral choroid plexus (CP) following
+10°C heat shock or zinc exposure in the presence and absence of
trimethylamine oxide (TMAO). Levels of Hsp70 mRNA were compared in non-treated
and stressed lateral CP tissues obtained from the same shark. +10°C heat
stress: tissue segments were incubated in medium with and without TMAO at
13.5°C for 7.5 h (
) or at 23.5°C for 1 h then 13.5°C for 1.5
h (+10°C). Zinc exposure/recovery: tissue segments were incubated at
13.5°C in medium with and without TMAO for 7.5 h without zinc (No Zn) or
with 50 µmol l–1 ZnSO4 for 6 h then without
zinc for an additional 1.5 h (Zn/Rec). Relative levels of hsp70 mRNA in
non-treated and stressed tissues were analyzed by semi-quantitative RT-PCR.
Shown here is an EtBr-stained 1.5% agarose gel on which aliquots of PCR
products from representative sets of heat-stressed and zinc-exposed tissue,
along with a DNA ladder, were electrophoresed; N=2–3 for each
experiment.
|
