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Fig. 5. Whole cell voltage-dependent currents in muscle fibres from honeybee. (A)
In voltage-clamp mode, in the presence of Tyrode's solution (2 mmol
l1 Ca2+) as the extracellular solution, a series
of depolarizations bringing the membrane potential from 80 mV to
30, 10, +10 and +30 mV over a period of 200 ms activated both
inward and outward currents. (B) In a fibre depolarized to +10 mV for 100 ms,
tetrodotoxin (TTX) had no obvious blocking effect on the inward current
(middle trace). However, Cd2+ and La3+ completely
blocked the inward component of the voltage-activated currents (lower
trace).