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Fig. 1. Single skeletal muscle fibres from adult honeybee leg muscle. (A)
Micrographs of two cells bathed in Tyrode's solution taken using phase
contrast (transmitted light) microscopy. (B) Part of a cell at a highest
magnification, (in horizontal orientation). (C–F) Laser confocal
micrographs of T-tubule system in isolated fibres. Single fibres bathed in
Tyrode's solution were stained with the lipophilic fluorescent dye di-8 ANEPPS
(10 µmol l–1) for 20 min in order to reveal the transverse
tubule network. (C) In longitudinal axial section, a central chain of nuclei
interrupting T-tubules (double arrow) and tracheoles (arrow) are visible. (D)
Longitudinal paraxial section, shows longitudinal connections between
T-tubules (arrows) within the same or adjacent sarcomeres. The dotted line (i)
shows the position at which the transverse fibre reconstruction shown in F was
taken. (E) Continuity of T-tubules with the surface membrane. Two T-tubules
per sarcomere penetrate the fibre volume, as emphasized by the profiles of
pixel intensity (below) taken at positions ii and iii. (F) Transverse confocal
section showing the shape of the fibre. (G) Another cylindrical fibre stained
with the calcium fluorophore Fluo-3-AM. Scale bars, 100 µm (A); 20 µm
(B); 20 µm (C,D,F,G); 4 µm (E).
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