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Figure 3


Fig. 3. Effect of the selective inhibitor SB203580 on the p38-MAPK and Hsp27 phosphorylation induced by CuCl2. Protein (50 µg, top and bottom, and 100 µg, middle) from hearts perfused without or with 500 µmol l–1 CuCl2 for 15 min in the absence or presence of 1 µmol l–1 SB203580 was analysed by immunoblotting with phosphospecific anti-p38-MAPK (top), phosphospecific anti-Hsp27 (middle) or anti-actin (bottom) antibodies. Western blots shown are representative of four independent experiments performed with similar results.





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