spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Phosphorylation of p38-MAPK and Hsp-27 by CuCl2. (A,C) Protein (A, 50 µg or C, 100 µg) from Rana ridibunda hearts perfused in the absence (Con) or presence of increasing concentrations of CuCl2 (50–500 µmol l–1) for 15 min was analysed by immunoblotting with anti-p38-MAPK (Ai) and anti-Hsp27 (C) phosphospecific antibodies. As a positive control, extracts from hearts perfused with 50 µmol l–1 H2O2 for 2 min were included. Identical samples were assayed with an anti-actin antibody as a control for protein loading (Aii). (B,D) Densitometric analysis of phospho-p38-MAPK (B) and phospho-Hsp27 (D) bands, by laser scanning. Results are means ± s.e.m. for three independent experiments. *P<0.05, **P<0.01, {dagger}P<0.001 vs control value.





Right arrow Return to article