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Fig. 5. Double-stained immunohistochemistry of serotonin (red) and synaptotagmin
(green). (A) In intact larva, serotonin was synthesized at the apical ganglion
(red) and synaptotagmin-possessed nerve cell network lined along the ciliary
band (green). (B) In pCPA-treated larva, the apical ganglion did not
produce serotonin, but formation of the synaptotagmin-possessing nerve cell
network (green) was not affected (B). Bar, 100 µm.