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Figure 3


Fig. 3. (A–G) Immunohistochemical localization of serotonin receptor cell processes (fibers) inserted into larval ectoderm. In plutei at 48 h post-fertilization (h.p.f.), fibers from serotonin receptor cell network are inserted into the ectoderm around the apical ganglion (Ab,B, arrows, H), extending from the oral lobe transverse tract (`oltrt' in B,H), at the left and right corners of oral lobe (Ac,C,H), at the tip of left and right arms (Ad,D,H), at the middle region of posterior body on both left and right sides (Ae,E,H), at ventral ciliary band (Af,F, arrows, H), and at the tip of posterior end of the body (Ag,G, arrow, H). Bars, 100 µm (A,h), 30 µm (B,E,F), 40 µm (C,D) and 20 µm(G). (H) Schematic ventral view of 48 h.p.f. pluteus larva summarizing major tracts of the serotonin receptor cell network and the sites where fibers are inserted into the ectoderm (green lines), based on present and previous observations (Katow et al., 2004). Letters b–g in gray rectangles correspond to the white rectangles in A and insets in C–G. This scheme shows tracts of serotonin receptor network shown by whole-mount immunohistochemistry (Ah), including left and right oral rod tracts (lort and rort), left and right anal rod tracts (lart and rart), dorsal stomach plexus (dsp) and dorsal central tract (dct). Digestive tracts are shown with light brown line.





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