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Figure 1


Fig. 1. Sequence of 5HThpr gene and protein and expected secondary structure. (A) 5HThpr gene consists of 2194 nucleotides comprising 543 bp of 5' untranslated region (UTR), 1395 bp of coding region and 254 bp of 3' UTR. The open reading frame encoded 465 amino acids that contained seven predicted transmembrane domains (shaded boxes) and an expected G-protein-coupled signature domain (underlined). Three potential N-glycosylation sites were shown (open boxes). (B) Schematic presentation of predicted secondary structure of 5HThpr based on analysis by SOSUI software program. First six transmembrane domains (1–6) from the N terminus (NH2) are primary helix, and seventh domain (7) nearest to the C terminus (COOH) is a secondary helix. A G-protein-coupled signature 1 locates between the third and fourth transmembrane domains. Cytoplasmic, inside the cell; extracellular, outside the cell.





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