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Fig. 5. The effect of removing endogenous NO by bath application of the NO
scavenger PTIO. (Ai) A 10 min bath application of 0.5 mmol
l–1 PTIO caused a statistically significant decrease in cycle
frequency (t=2.04, *P<0.05, N=11). A
30 min wash with locust saline resulted in the digging rhythm returning to its
original cycle frequency. (Aii) An example showing that a 10 min bath
application of 0.5 mmol l–1 PTIO resulted in a decrease in
the number of cycles within the same time period.