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Fig. 3. The effects of the NO donor SNAP on oviposition digging. (Ai) A 10 min bath
application of SNAP resulted in a statistically significant increase in the
cycle frequency of the digging rhythm (t=2.5,
*P<0.05, mean ± s.e.m. of five animals). (Aii)
An example showing the effect of increased exogenous levels of NO on the
digging rhythm. A 10 min bath application of 0.2 mmol l–1
SNAP resulted in an increase in the number of cycles of muscle activity while
a 30 min wash with locust saline resulted in the number of cycles of muscle
activity returning to control. (B) The effect of bath application of the
inactive isomer of SNAP, NAP, on the cycle frequency. A 10 min bath
application of 0.2 mmol l–1 NAP had no effect on the cycle
frequency (t=0.14, P>0.05, N=4). (C) A 10 min
bath application of 0.2 mmol l–1 degassed SNAP (DG SNAP) had
no significant effect on cycle frequency (t=0.17, P>0.05,
mean ± s.e.m. from four animals). (D) Bath application of normal locust
saline similarly resulted in no significant effect on cycle frequency
(t=–1.81, P>0.05, mean ± s.e.m. from four
animals).