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Fig. 4. Western blot showing impacts of temperature and juvenile hormone (JH) on
JH-binding by various proteins. All colors and labeling correspond identically
to that described for Fig. 3.
Blots were probed with anti-JH antiserum, which directly enables
identification of JH covalently bound to denatured proteins. Three key
proteins were identified by the anti-JH, including the Hexamerin-1 (Hex-1)
protein, an unknown 100 kDa protein and another unknown group of proteins in
the 50 kDa range. Molecular mass markers were pre-stained and visible before
immunodetection.
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