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Figure 6


Fig. 6. Representative pictures of killifish lamellar cross-sections, labeled with anti-proendothelin-1 (brown) and anti-NKA (blue). (A,B) Gill sections from a chronic (>30 day) FW acclimated killifish and (C,D) a chronic SW acclimated killifish. (B,D) Sections were incubated in normal goat serum as a negative control for proendothelin immunoreactivity, and doubled labeled with anti-NKA (blue) to illustrate the position of the mitochondrion-rich cells (MRC) in the gill. Proendothelin immunoreactivity was found in a cell adjacent to the NKA immunoreactivity (MRC) and on gill pillar cells in both the FW and SW killifish. The immunoreactivity presented here matches the in situ hybridization of mRNA probes for EDN1s and NKA in Fig. 5. Scale bars, 50 µm.





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