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Fig. 2. High-speed microscopy was used to track the position of the glass fiber
used for stiffness measurements. (A) Individual larvae were inserted into a
bed of agar with their tails pinned beneath a dull probe (triangle) under the
objective lens of a fixed-stage compound microscope. When held in this
position, the tip of a glass fiber was pressed against the middle of an
individual neuromast (inset). (B) During experiments, the position of the
fiber was recorded with a high-speed video camera mounted on the microscope.
Video recordings were analyzed to track the movement of the edge of the glass
fiber. A single video frame from one of these recordings is shown with the
edge (purple line) that was found for the prescribed pixel stripe. (C) The
pixel intensity along this stripe is shown with a curve fit (green line). (D)
The first derivative of the fitted line with respect to position was used to
find the position of maximum change in pixel intensity. This point was
interpreted as the edge of the glass fiber.
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