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Figure 7


Fig. 7. Energy transfer between Pdc and Gβ{gamma} in Blepharisma detected by the FRET Sensitized Emission Method. Pixel by pixel FRET values between Pdc labeled with AlexaFluor 546 (A1, B1) and Gβ labeled with AlexaFluor 488 (A2, B2) in dark-adapted (A1–A4) and in light-stimulated cells (B1–B4). FRET images (A4, B4) are pseudocolored relative to the amplitude of FRET efficiency. The overlay is red where the overlap is significant (B4). The color code ranges between 0% (violet) and 100% (red).





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