|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Coimmunoprecipitation of Pdc and 14-3-3 protein in Blepharisma.
Total cell lysates were subjected to immunoprecipitation with antibody against
Pdc. The precipitated proteins in lysates from dark-adapted cells (lane 1),
light-stimulated cells (lane 2) and cells incubated with okadaic acid prior to
illumination (lane 3) were resolved by SDS-PAGE and immunoblotted for 14-3-3
protein. Arrows mark a coimmunoprecipitated protein of 
33 kDa
(lanes 1–3). Western blot analysis confirmed that this polypeptide
represents a putative 14-3-3 protein (lane 4). Lower panel shows a
densitometric quantification diagram of 14-3-3 protein levels in cell
lysate under different experimental conditions (lysate from dark-adapted or
illuminated cells and cells incubated with okadaic acid prior to illumination,
respectively). The level of 14-3-3 protein in lysate from the control
dark-adapted cells was taken as 100%. Bars represent the mean values ±
s.e.m. of three experiments.
|
