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Figure 1


Fig. 1. Immunodetection of phosphorylation level of ciliate Pdc and interaction of Pdc with Gβ{gamma} in Blepharisma. The precipitated proteins with specific antibody against Pdc in lysates from dark-adapted cells (lane 1), light-stimulated cells (lane 2) and cells incubated with okadaic acid prior to illumination (lane 3) were analyzed by western immunoblotting with antibodies against phosphoserine (A) or Gβ (B). Lanes 4 and 5 show immunoblots identifying Gβ{gamma} in a whole cell lysate from Blepharisma and the rod outer segment (ROS) of bovine photoreceptor cells, respectively (B). The blots are representative of the results of three independent experiments. Inserted densitometric diagrams show the levels of Pdc phosphorylation (A) and bound Gβ (B) in lysates from dark-adapted cells and cells exposed to light or okadaic acid. The levels of Pdc phosphorylation or bound Gβ in lysate from the control dark-adapted cells were taken as 100%. Bars represent mean values ± s.e.m. of three experiments.





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