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Fig. 2. Calibration curve using three different test tubes (with water added) of
known mass. Test tubes have the same diameter (0.01135 m), but were cut to
different heights to reduce their mass, 0.52 g (*), 1.1 g (o), and
2.6 g (x). A linear relationship exists for the larger test tube (x), but does
not extend into the range of pixel areas around worms, indicated by the
horizontal dotted lines. We instead used a second-order polynomial fit through
the medium-sized test tube (o) that covered the range of observed pixel areas
around worms (r2=0.997, N=11).
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