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Fig. 7. Immunolocalization of PKC and phospho-PKC substrates on sea urchin
spermatozoa. The presence of PKC and its phosphorylated substrates were
evidenced by immunofluorescence with an anti-phospho-PKC antibody (p-PKC pan)
and an anti-phospho-PKC substrate (p-PKC subst.) antibody in both immotile and
motile spermatozoa (central columns). Labeling of flagellar ß-tubulin
with a specific monoclonal antibody (see Materials and methods) is also shown
for comparison purposes. In controls without any primary antibody
(2nd Ab only), a weak spot of fluorescence was present at the base
of sperm heads. The outer columns show similar images to those in the middle
columns but viewed by phase-contrast. Scale bars, 10 µm.
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