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Fig. 6. PKC phosphorylation change following the initiation of motility in intact
sea urchin spermatozoa. The presence of PKC and its catalytic cleavage product
(arrowheads) was evidenced by immunoblotting with an anti-phospho-PKC antibody
(pan). The protein phosphorylation profile obtained with the anti-phospho-PKC
substrate antibody (subs) is presented for comparison. i, immotile; m, motile.
A blot representative of two other blots is presented.