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Fig. 4. Conditioning protocol also affects recall after single-trial conditioning.
The proportion of subjects responding to the first recall test with the
rewarded odor immediately (NAPC=35;
NAC=40; NPC=33;
NUNP=31), 24 h (NAPC=39;
NAC=25; NPC=42;
NUNP=29) or 96 h (NAPC=48;
NAC=75; NPC=30;
NUNP=22) after conditioning is shown. None of the honey
bees trained with the unpaired protocol responded on the immediate recall
test. Recall at each time post-conditioning depended upon the conditioning
protocol (lreg;
23=39.6, P<0.001).
Recall of the odor CS decays for all conditioning protocols (APC, PC, AC) over
a period of 96 h (lreg;
22=6.27,
P=0.043). Recall for AC, PC and UNP was compared to that for APC
using a least-squares contrast at each time point; * indicates a
difference of P<0.05.