Supplemental Figure S1 (Adobe PDF) -
Fig. S1. Representative non-Gaussian frequency distributions of the area (A), perimeter (B), form factor (C), roundness (D) and aspect ratio (E) of the colloid of all individual thyroid follicles in the subpharyngeal region, head kidney and kidney of carp. Graph inserts represent the boxed area, and highlight the mode of the frequency distributions. To avoid subjective selection of bin width and endpoint, the frequency distribution was determined by kernel density estimation using an add-in utility (version 1.0e) for Microsoft® Excel from the Royal Society of Chemistry.