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Fig. 3. Surgical procedure for topical microinjection of kainic acid in seawater
(SW) eels. (A) Exposed skull of SW eel (left) and corresponding loci of eel
brain (right). The drill hole was made around supraoccipital bone to expose
the area postrema (AP). Average size of the AP is approximately 0.8 mm. (B)
Experimental setup for injection of 50 nl of kainic acid into the AP of SW
eels. A glass capillary was connected to the manipulator to facilitate
accurate placement of the capillary at the AP. The capillary was also
connected to the microsyringe, through which 50 nl of 4 mmol
l–1 kainic acid containing 1% Sky Blue 6B in PBS is injected.
(C) Distribution of Chicago Sky Blue 6B in eel brain (left, AP-lesioned eel;
right, lesion control). Area of the AP is circled by broken line. TeO, optic
tectum; Cb, cerebellum; IV, fourth ventricle.
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