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Figure 8


Fig. 8. Immunohistochemistry of AgCA9 protein in whole-mount larvae (A,C,D) and longitudinal paraffin sections of larvae (B). The figure shows laser confocal microscope images of An. gambiae larval rectum immuno-stained with chicken antibodies 5340 (green; A,D) and 5563 (green; B) and mouse monoclonal antibodies against Na+/K+-ATPase (red; B,C). The ileum is indicated at the anterior end of the rectum (A,C,D). AgCA9 protein localized to the DAR cells exclusively (A,B,D; arrow). The banding pattern seen in the DAR cells of whole mounts of the rectum is due to the circumferential muscles of the rectum. This is better seen in D, which shows muscle stained with phalloidin (red). Na+/K+-ATPase localized to the entire rectum, with the exception of the DAR cells (B,C). DAR cells are indicated with an arrow in each image. Scale bars: 150 µm (A,C); 75 µm (B,D).





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