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Fig. 4. qPCR analyses of AgNHA1 transcript expression in fourth instar larval
tissues of An. gambiae. Data are presented as the mean + s.e.m. of
three averaged replicates of three independent experiments. An.
gambiae 18S ribosomal RNA was used as a reference gene. Results were
normalized to values for carcass at one. VNC, ventral nerve cord; SG/cardia,
salivary glands and cardia; GC, gastric caeca; AMG, anterior midgut; PMG,
posterior midgut; MT, Malpighian tubules.