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Fig. 3. An illustration of the methodology employed to estimate the diffusion
coefficient values of bulk sperm (D value). The processed image
showing diffused bulk sperm is divided into small rectangular zones (gray
rectangles) of 29.5–31.5 µm width. The average light intensity of
each rectangular zone (I value) and the location of its center along
the length of the channel (x value) are recorded. The I
values are normalized (called In values and shown in the
figure) using the intensity value of the corresponding sample
(Io value). The x values as X
coordinates and In values as Y coordinates are
supplied to the MATLAB curvefitting toolbox. The D value is obtained
by curve-fitting Eqn 6 to the
In–x data. Not all In
and x values are shown for the sake of clarity. erf, error
function.
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