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Figure 6


Fig. 6. Effect of uncaging inositol-trisphosphate (IP3) on cytosolic Ca2+ and Na+. (A) Uncaging IP3 with a UV pulse elicited a cytosolic Ca2+ rise ({Delta}F) but no membrane potential change. A second UV pulse changed neither cytosolic Ca2+ nor membrane potential, and membrane depolarization in 40 mmol l–1 K+ still evoked a normal transient Ca2+ rise. (B,C) Summary of effects of uncaging IP3 and of 40 mmol l–1 K+ on cytosolic Ca2+ (B) and on the membrane potential (C). (D,E) Uncaging of IP3 elicited a reversible intracellular Na+ rise but no membrane potential change. A second UV pulse had no effect on either Na+ or membrane potential. *P<0.05.





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