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Fig. 8. Actin distribution and morphology of pinacocytes. (A) Scanning electron
microscopy shows that endopinacocytes (enp) are elongated cells that form the
underside of the apical pinacoderm. Dotted lines indicate cell boundaries.
Scale bar, 10 µm. (B) Epifluorescence microscopy of Bodipy 505 FL
Phallacidin-labelled tissue shows extensive tracts of actin in endopinacocytes
of the apical pinacoderm, a region equivalent to that shown in A. Actin is
brightly labeled in focal adhesion plaques between cells (arrows). Dotted
lines indicate cell boundaries, demonstrating that the actin tracts continue
in adjacent cells. Scale bar, 50 µm. (C) In cells lining excurrent canals
of a sandwich preparation, actin tracts (black arrows) are much less brightly
labelled. The preparation was fixed as a wave of contraction passed through
the field of view. Dense packing of choanocyte chambers (cc) indicates that
the lower canal was contracted. Scale bar, 100 µm.
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