|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. (A) Side-view of the experimental set-up for imaging sperm-egg interactions
(not drawn to scale). Here, the Taylor-Couette apparatus is mounted vertically
for use in fertilization experiments. It consists of two nested cylinders,
with an 8 mm wide seawater-filled gap between them. Rin
and Rout refer to the radii of the inside (6.1 cm) and
outside (6.9 cm) cylinders, respectively. Counter-rotation of the two
cylinders produces a steady laminar flow over a wide range of shears
(0–10 s–1) at low Reynolds numbers
(Re<150). Further details are provided in the text. (B) Top view
of the seawater gap between vertically mounted cylinders with arrows denoting
flow velocity vectors. A cross-over point of no translational velocity occurs
between the counter-rotating flows. (C) A sequence of overlaid, digitized
images (at 0.1 s intervals) of a single sperm, as it slips past an egg at 2
s–1. The egg is positioned at the cross-over point, and thus
remains stationary, while rotating from right to left. Bar, 100 µm.
|
