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Figure 9


Fig. 9. Stimulation of chitin synthesis in midgut extracts by serine proteases. Relative chitin synthase activity was measured by the incorporation of N-acetyl-D-[U-14C]glucosamine into alkali-insoluble material obtained from crude extracts of the anterior midgut of fifth instar larvae. (1) Activities of untreated extracts (0.4 nmol mg–1 h–1) were set to 100%; (2–4) relative activities in the presence of trypsin at a concentration of 10 µg µl–1 (2), chymotrypsin at a concentration of 10 µg µl–1 (3), and 5 mmol l–1 Pefabloc SC serine protease inhibitor (4). Mean values (±s.e.m.) from three independent experiments are given as a percentage of the control.





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