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Figure 3


Fig. 3. ANCE is localised to the giant vesicles of the secondary cells of the male accessory gland (AG). (A) Immunofluorescence staining of ANCE in the secondary cells of the distal region of the male AG using ANCE-specific primary antiserum and FITC-conjugated secondary antibodies. Giant vesicles are numbered clockwise and the two nuclei (N) are labelled. (B) Three-dimensional representation of the vesicles (red) in the secondary cell (green) of the AG. Serial confocal sections were collected through the sample, with an optical depth of 1 µm. One of these sections is shown. (C) Electron micrograph of a giant vesicle of an AG secondary cell showing the dense core material (star) and filaments (f). Scale bar, 2 µm.





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