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Files in this Data Supplement:
Fig. S1. Results of the training and transfer tests at mazes A and B with sector and ring patterns. (a) Results of the training tests at mazes A and B with yellow patterns in the morning and blue patterns in the afternoon. At maze B in the morning, the choice frequency for the yellow sector pattern was 0.80±0.03. At maze A in the afternoon, the choice frequency for the ring pattern was 0.85±0.02. Results of ANOVA tests are as follows: d.f. (interaction)=4, F=0.266, P=0.891; d.f. (time)=1, F=329.9, P<0.0001; d.f. (repeated tests)=4, F=0.243, P=0.913. All the Bonferroni post tests showed performance in the morning and in the afternoon are significantly different. The bees clearly changed their preference from the sector pattern in the morning to the ring pattern in the afternoon (b) Results of the transfer tests with black patterns at mazes A and B. At maze B, the choice frequency for the sector pattern was 0.80±0.05 (N=14), while at maze A, the choice frequency for the ring pattern was 0.89±0.05 (N=14). Results of ANOVA tests are as follows: d.f. (interaction)=2, F=0.653, P=0.526; d.f. (time)=1, F=142.1, P<0.0001; d.f. (repeated tests)=2, F=0.067, P=0.935. All the Bonferroni post tests showed performance in the morning and in the afternoon are significantly different. The bees had clearly changed their preference from the sector pattern in the morning to the ring pattern in the afternoon (t-test, P<0.001). Values are means ± s.e.m.; N, number of individual bees attending the tests. See text for further details.
Fig. S2. Results of the transfer test at maze C with black sector and ring patterns. The choice frequency for the sector pattern in the morning was 0.55±0.07. In the afternoon, the bees chose the ring pattern with a frequency of 0.74±0.07. Results of ANOVA tests are as follows: d.f. (interaction)=1, F=0.000, P=0.96; d.f. (time)=1, F=12.1, P<0.01; d.f. (repeated tests)=1, F=0.111, P=0.741. All the Bonferroni post tests showed performance in the morning and in the afternoon are significantly different. The bees had significantly reversed their pattern preference between the morning and afternoon testing sessions. Values are means ± s.e.m.; N, number of individual bees attending the tests. See text for further details.
Fig. S3. Results of the control tests at mazes A and B. (a) At maze A, the choice frequency for the right side was 51.7%, while that for the left side was 48.3%. Thus, the bees did not have a preference for a particular side of maze A (2×2 McNemar test, P=0.961, N=58). (b) At maze B, the bees chose the right side in 52.8% of the visits. The left side was chosen in 47.1% of the visits. The bees did not have a preference for a particular side of maze B (2×2 McNemar test, P=0.936, N=70). Values are means ± s.e.m.; N, number of individual bees attending the tests. See text for further details.
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