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Fig. 1. for affects food intake. (A) Mid-third-instar rover (for^R), sitter (for^s) and sitter mutant (for^s2) larvae with ingested dye visible through the ventral cuticle. (B) Well-fed rovers (for^R) had significantly lower food intake than well-fed sitters after 10 min (ANOVA, F_(2,84)=12.03, P<0.0001), 15 min (F_(2,85)=11.46, P<0.0001) and 20 min (F_(2,85)=7.09, P<0.001) on dyed yeast paste. A two-way ANOVA on strain (for^R, for^s, for^s2) and time (10, 15, 20 min) showed a significant effect of strain (F_(2,254)=12.12, P<0.001), no effect of time (F_(2,254)=0.13, P=0.9) and no strain-by-time interaction (F_(2,254)=0.23, P=0.9). (C) Rovers had significantly less blue area than sitters at early third (F_(2,89)=4.24, P=0.02) and mid-third-instar (F_(2,87)=10.52, P<0.0001) stages. (D) Spectrophotometric quantification of homogenates from larvae fed a Erioglaucine (FD and C Blue No. 1) dyed yeast paste for 15 min showed that for^R larvae ingest significantly less dye than for^s or for^s2 larvae (F_(2,26)=7.88, P<0.01).

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