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Figure 6


Fig. 6. In vitro confirmation of the effects of 20E on ecdysteroid-responsive genes. Real-time RT-PCR relative quantification of two putative ecdysteroid-responsive genes, digestive cysteine proteinase 1 (A) and trypsin (B) in a 24 h hepatopancreatic tissue culture subjected to four different concentrations of 20E (1, 10, 100 and 1000 pg µl–1). Different letters indicate statistical significance (P<0.05).





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