|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Changes in gastrolith size during an induced molt cycle in a 20E-injected
male C. quadricarinatus. (A) Digital X-ray imaging of the gastrolith
in two representative specimens, one injected with 20E (bottom) and the other
with carrier (top), which served as control. Images were obtained in the
premolt stage on days –13, –6 and –4 relative to the
anticipated time of ecdysis; a round metal grid was used for size calibration
(10 mm diameter). (B) Upper graph shows the injection regime of 20E, (from a
stock solution: 1 µg µl–1 of a saline buffer containing
10% ethanol) injected twice a day; 1000 pg µl–1 was
calculated as the maximum physiological level. The injection regime and volume
of the carrier were identical in the control group and 20E-injected groups.
The x axis is normalized to days from ecdysis. Lower graph shows
changes in MMI during the induced molt cycle by repetitive injections of 20E.
The x axis is normalized to days from ecdysis. Squares, 20E-injected
animal; circles, carrier-injected control.
|
