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Figure 1


Fig. 1. Voltage-clamp recordings from cells in acetate or biotin. The cells were held at resting potential (-30 mV) using two-electrode voltage-clamp. (A) Solid bars indicate application of 1 mmol l-1 acetate. Lower two traces were recorded using K+-filled electrodes with 1 mmol l-1 K+ in the bath. K+ currents were suppressed in the middle and upper traces using CsCl and tetraethylammonium (TEA), leaving Ca2+ (1 mmol l-1) and Mg2+ (5 mmol l-1) as the predominant charge carriers. Arrows show the outward conductances upon addition of acetate and removal of acetate. (B) Solid bars indicate application of variable concentrations of biotin at variable pH. K+ currents were suppressed in the middle and upper traces using CsCl and TEA leaving Ca2+ (1 mmol l-1) and Mg2+ (5 mmol l-1) as the predominant charge carriers. Arrows show the inward conductances upon removal of biotin.





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