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Figure 4


Fig. 4. Changes in [Ca2+]i (A), pHi (B) and proton secretion rate (C) of trout hepatocytes upon exposure of cells to Ca2+-free medium containing 0.5 mmol l–1 EGTA followed by addition of 25 µmol l–1 of the intracellular Ca2+ chelating agent BAPTA-AM. The effect of exposure to 25 µmol l–1 BAPTA-AM in the presence of Ca2+e is depicted as BAPTA control in the three experiments. Data are means ± s.e.m. of 27–38 cells from 3–4 independent preparations in A and B and from three independent preparations in C.





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