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Fig. 2. Changes in pHi of trout hepatocytes following addition of 0.5 µmol
l–1 ionomycin (A) or 0.1 µmol l–1
thapsigargin (B) (at the time indicated by the arrows) using
Ca2+-containing medium, Ca2+-free medium and
Ca2+-free medium following incubation of cells with the
intracellular Ca2+ chelating agent BAPTA-AM (25 µmol
l–1). Data are means ± s.e.m. of 19–128 cells
from 3–16 independent preparations in A and of 28–39 cells from
3–4 independent preparations in B.