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Fig. 6. Tissue distribution of the three alternatively spliced transcripts of
FoxK1 in adult T. rubripes. Relative expression levels of
TFoxK1-
(blue bars), TFoxK1-
(yellow bars) or
TFoxK1-
(red bars) in fast muscle (WM), slow muscle (RM),
heart (H), liver (L), skin (SK), brain (B) and gonads (G) are represented as
ratios against the expression level of TFoxK1- in fast muscle.
RNA polymerase II was used as standard. Values are means ± s.e.m.
(N=4). Statistically significant differences (P<0.05)
between splice variants within a tissue are labelled with the same letter;
significant differences in expression of an isoform across different tissues
are indicated by the same number. (Inset) Expression of FoxK1- in fast
myotomal muscle of adult fish. Transverse sections of fast muscle were
immunostained for FoxK1-, a molecular marker of muscle progenitor cells
(MPCs). FoxK1--positive nuclei (indicated by arrowheads) occupy a
sublaminar position that is typical of MPCs.
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