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Fig. 1. (A) Reference spectra depicting the millimolar extinction coefficients of
zebrafish oxygenated hemoglobin (oxyHb), methemoglobin (metHb),
nitrosylhemoglobin (HbNO) and deoxygenated hemoglobin (deoxyHb) as function of
wavelength at pH 7.3. (B) Representative result from the nonlinear
least-squares curve-fitting procedure. The black curve shows the experimental
spectrum of the diluted hemolysate from a nitrite-exposed zebrafish, and the
red curve gives the fitted curve. The estimated millimolar concentrations of
oxyHb, metHb, HbNO and deoxyHb in the cuvette, and the error on the parameter
values, are shown in the insert.
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